Skip to main content
| | | Screen Reader Access |   
   |   
  • Photoprotective sulfated mannogalactan from heterotrophic Bacillus velezensis blocks UV-A mediated matrix metalloproteinase expression and nuclear DNA damage in human dermal fibroblast.

    Prolonged exposure of human dermal fibroblasts (HDF) to ultraviolet (UV) radiation triggers the production of reactive oxygen species by upregulating the expression of matrix metalloproteinases (MMPs), causing type-I collagen degradation and photoaging. A sulfated (1 → 3)/(1 → 4) mannogalactan exopolysaccharide (BVP-2) characterized as [→3)-α-Galp-{(1 → 4)-α-6-O-SO3-Manp}-(1 → 3)-α-6-O-SO3-Galp-(1→] was isolated from seaweed-associated heterotrophic bacterium Bacillus velezensis MTCC13097. Whole genome analysis of B. velezensis MTCC13097 (Accession number JAKYLL000000000) revealed saccharine biosynthetic gene clusters for exopolysaccharide production. BVP-2 administered cells showed noteworthy reduction in mitochondrial superoxide (∼85 %, p < 0.05) and ROS production (62 %) than those exhibited by UV-A irradiated HDF cells. Oxidative imbalance in HDF cells (after UV-A exposure) was recovered with BVP-2 treatment by significantly downregulating nitric oxide (NO) production (98.6 μM/mL, 1.9-fold) and DNA damage (?67 %) in comparison with UV-A induced cells (191.8 μM/mL and 98.7 %, respectively). UV-irradiated HDF cells showed a ∼30-50 % downregulation in the expression of MMPs (1, 2, and 9) following treatment with BVP-2. Considerable amount of sulfation (18 %) along with (1 → 3)/(1 → 4) glycosidic linkages in BVP-2 could be pivotal factors for down-regulation of the intracellular MMP-1, which was further supported by molecular docking and structure-activity studies. The (1 → 3)/(1 → 4)-linked bacterial exopolysaccharide (BVP-2) might be used as prospective natural lead to attenuate and mitigate UV-A-induced photoaging.

     

    Read more...

  • Mitogenomic recognition of incognito lineages in the mud spiny lobster Panulirus polyphagus (Herbst, 1793): A tale of unique genetic structuring and diversification
  • Spatiotemporal analysis in brown seaweed diversity and abundance at selected coastal locations on the Southern coast of Tamil Nadu, India: A way forward for conservation and sustainable utilization
  • New Record of Fanged Seabream, Sparidentex jamalensis (Perciformes: Sparidae) From Indian Waters
  • Spatial variation of the broodstock availability of Acanthopagrus berda (Forsskal 1775) with emphasis on seasonality, lunar periodicity, and sex ratio for facilitating efficient
  • In vitro culture and characterisation of a new brain cell line from the spine cheek anemone fish Premnas biaculeatus
  • Insights into ovarian maturation and reproductive traits of the deep-water penaeid shrimp Metapenaeopsis andamanensis (Wood-Mason in Wood-Mason and Alcock, 1891)
  • img
  • img
  • img
  • img
  • img
  • img
  • img
  • img
  • img
  • img

Search...